Method Development for the Chromatographic analysis of a Two-Component Tablet Formulation Using Chemometric Optimization Technique

A new ultra-performance liquid chromatography (UPLC) method was improved for the determination of sulfamethoxazole and trimethoprim in a two-component tablet formulation. In development UPLC method, chromatographic conditions were chemometrically optimized by using experimental design optimization procedure. The central composite fitting model applied to identify suitable providing desirable elution chromatogram. design, temperature, flow rate buffer% selected as effective factors on resolution. buffer system mixture 0.1 M CH3COOH CH3COONa (pH 4.75) mobile phase system. step, found be 0.20 mL/min rate, 38.0 °C column temperature 66% acetate (v/v) phase. Separation analyzed compounds performed Waters BEH C18 (100 mm-2.1 mm, 1.7 mm i.d.). validation recovery study applying synthetic binary trimethoprim. Recovery results 99.12% 99.44% chemometric quantitation commercial tablets.